{"id":1843,"date":"2023-06-21T09:13:03","date_gmt":"2023-06-21T01:13:03","guid":{"rendered":"https:\/\/ciderhousetest.com\/labnetwest26\/?p=1843"},"modified":"2025-12-22T06:03:51","modified_gmt":"2025-12-21T22:03:51","slug":"effect-of-temperature-and-ph-on-2-enzymes-amylase-and-pepsin","status":"publish","type":"post","link":"https:\/\/ciderhousetest.com\/labnetwest26\/effect-of-temperature-and-ph-on-2-enzymes-amylase-and-pepsin\/","title":{"rendered":"Effect of Temperature and pH on 2 Enzymes: Amylase and Pepsin"},"content":{"rendered":"\n<h4 class=\"wp-block-heading\">pH<\/h4>\n\n\n\n<p>Each enzyme has an\u00a0optimum pH, above or below which their activity is lower.<\/p>\n\n\n\n<p>Supply students with 5 ph buffers i.e\u00a0\u00a0ph 2,4,7,10 and 12<\/p>\n\n\n\n<p>Activity was measured by transmittance\u00a0 and absorbance\u00a0using a Mystrica colourimeter\u00a0<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Temperature:<\/h4>\n\n\n\n<p>All reactions\u00a0are faster at a higher temperature, however the enzyme-catalyzed reactions become slower or stop if the temperature is too low or\u00a0too high, because\u00a0enzymes become\u00a0denatured at high temperature. \u00a0Set up an ice bath, a room temperature\u00a0water bath (about 22\u00b0C), a water bath at body temperature\u00a0(37\u00b0C), one at 50\u00b0C and one at 75\u00b0C<\/p>\n\n\n\n<p>The students add 2ml of enzyme\u00a0and 2 ml of solution in different test tubes and left them at the different temperatures.\u00a0<\/p>\n\n\n\n<p>After 5 minutes they check for differences by using a Mysterica colourimeter<\/p>\n","protected":false},"excerpt":{"rendered":"<p>pH Each enzyme has an\u00a0optimum pH, above or below which their activity is lower. Supply students with 5 ph buffers i.e\u00a0\u00a0ph 2,4,7,10 and 12 Activity was measured by transmittance\u00a0 and absorbance\u00a0using a Mystrica colourimeter\u00a0 Temperature: All reactions\u00a0are faster at a higher temperature, however the enzyme-catalyzed reactions become slower or stop if the temperature is too [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[31],"tags":[],"class_list":["post-1843","post","type-post","status-publish","format-standard","hentry","category-technical-tips"],"_links":{"self":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts\/1843","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/comments?post=1843"}],"version-history":[{"count":0,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts\/1843\/revisions"}],"wp:attachment":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/media?parent=1843"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/categories?post=1843"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/tags?post=1843"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}