{"id":1896,"date":"2023-11-20T07:46:26","date_gmt":"2023-11-19T23:46:26","guid":{"rendered":"https:\/\/ciderhousetest.com\/labnetwest26\/?p=1896"},"modified":"2025-12-22T06:03:51","modified_gmt":"2025-12-21T22:03:51","slug":"agar-diffusion-practical-suggestions-collated","status":"publish","type":"post","link":"https:\/\/ciderhousetest.com\/labnetwest26\/agar-diffusion-practical-suggestions-collated\/","title":{"rendered":"Agar Diffusion Practical suggestions (collated)"},"content":{"rendered":"<ol style=\"list-style: lower-alpha;\">\n<li>Dabco trays (see below), work well because of the use of the divider.<br \/>\n<a href=\"https:\/\/www.haines.com.au\/dabco-unitray-with-insert-10-place.html\" target=\"_blank\" rel=\"noopener\">Dabco Unitray with insert \u2013 Haines Educational<\/a><\/li>\n<li>Use Dabco trays as a mould for the agar and then just cut it into size.<\/li>\n<li>Use bromothymol blue and vinegar. I can send you the practical sheet and tech notes. Unitrays with the divider removed will give you 3cm Agar if filled to the top.<br \/>\nJust tap the set agar out of the Unitray once set and cut the 3cm3, 2 cm3 and 1cm3 using a scalpel and ruler. Tip \u2013 for this recipe, use tap water NOT distilled. 20g agar in 1 litre tap water. 20 squirts bromothymol blue (green-blue colour), heat\/ boil and pour into Unitray. Once placed in the vinegar, the colour changes to yellow.<\/li>\n<li>Use different sized silicon ice trays or a Unitray. Use a sharp knife to cut if needed, worked well.<\/li>\n<li>Use fishing line to cut the cubes. Make one large block of agar in a tray \/ container you have available e.g. a reagent tray that is normally used to store dropper bottles. Turn out the set agar onto a piece of laminated graph paper to measure up whatever size cubes you need.<\/li>\n<li>The Dabco trays, then cut the agar with a scalpel blade.<\/li>\n<li>Use Dabco trays (used for storing dropper bottles) when making the coloured agar.<br \/>\nFill one completely full, the second half full and the third about a quarter full.<br \/>\nOnce set remove the agar with a gentle tap on the bottom.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Dabco trays (see below), work well because of the use of the divider. Dabco Unitray with insert \u2013 Haines Educational Use Dabco trays as a mould for the agar and then just cut it into size. Use bromothymol blue and vinegar. I can send you the practical sheet and tech notes. Unitrays with the divider [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6,4],"tags":[],"class_list":["post-1896","post","type-post","status-publish","format-standard","hentry","category-advice","category-experiments"],"_links":{"self":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts\/1896","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/comments?post=1896"}],"version-history":[{"count":0,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts\/1896\/revisions"}],"wp:attachment":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/media?parent=1896"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/categories?post=1896"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/tags?post=1896"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}