{"id":1915,"date":"2023-11-20T08:05:31","date_gmt":"2023-11-20T00:05:31","guid":{"rendered":"https:\/\/ciderhousetest.com\/labnetwest26\/?p=1915"},"modified":"2025-12-22T06:03:50","modified_gmt":"2025-12-21T22:03:50","slug":"methyl-cellulose-suspension","status":"publish","type":"post","link":"https:\/\/ciderhousetest.com\/labnetwest26\/methyl-cellulose-suspension\/","title":{"rendered":"Methyl Cellulose Suspension"},"content":{"rendered":"<p>(This is used to slow down protozoa for microscope):<\/p>\n<p><strong>Method 1:<\/strong><br \/>\nHeat is needed to dissolve and make a suspension of the Methyl cellulose powder,\u00a0but it is important to have icy cold water on hand to cool the mixture down and stir fast!<\/p>\n<p>3% aqueous Methyl cellulose:<\/p>\n<ul>\n<li>Heat 100ml of deionised (DI) water to 85\u00b0C<\/li>\n<li>Shake 3g of Methyl Cellulose powder into the hot water and stir rapidly while cooling the solution to 5\u00b0C in an ice bath.<\/li>\n<li>The solution is stable at room temperature but store in tightly closed containers<\/li>\n<\/ul>\n<p><strong>Method 2:\u00a0<\/strong><br \/>\nFrom: researchgate.net<\/p>\n<p>4% Methyl cellulose<\/p>\n<ul>\n<li>Take 1\/2 the volume of your liquid, heat to about 300C in a vessel with a large stir bar. Use a 200 mL wide-mouth glass tissue culture bottle if available.<\/li>\n<li>Put the other 1\/2 volume of liquid in a container on ice to cool to 1-4 deg C.<\/li>\n<li>Take the WARM solution off the heat, add your Methyl cellulose whilst stirring.\u00a0 Manual stirring with a glass rod works best. The Methyl cellulose will not go into solution yet; it will be white and sticky with blobs.<br \/>\nNow add your COLD solution, put on a stir plate and stir vigorously.\u00a0 You may have to stir a few times manually to loosen the stir bar.\u00a0 The stir plate must have a strong magnet to generate enough force.\u00a0 If it is too weak, stir by hand.<\/li>\n<li>Once most of the Methyl cellulose is in solution, refrigerate overnight.\u00a0 Bubbles will disappear and you will have a clear solution.<br \/>\nExcess heat causes methylcellulose to gel, which will lead to cloudiness.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>(This is used to slow down protozoa for microscope): Method 1: Heat is needed to dissolve and make a suspension of the Methyl cellulose powder,\u00a0but it is important to have icy cold water on hand to cool the mixture down and stir fast! 3% aqueous Methyl cellulose: Heat 100ml of deionised (DI) water to 85\u00b0C [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[31],"tags":[],"class_list":["post-1915","post","type-post","status-publish","format-standard","hentry","category-technical-tips"],"_links":{"self":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts\/1915","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/comments?post=1915"}],"version-history":[{"count":0,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts\/1915\/revisions"}],"wp:attachment":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/media?parent=1915"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/categories?post=1915"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/tags?post=1915"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}