{"id":2053,"date":"2025-04-30T13:47:49","date_gmt":"2025-04-30T05:47:49","guid":{"rendered":"https:\/\/ciderhousetest.com\/labnetwest26\/?p=2053"},"modified":"2025-12-22T06:03:50","modified_gmt":"2025-12-21T22:03:50","slug":"methyl-cellulose","status":"publish","type":"post","link":"https:\/\/ciderhousetest.com\/labnetwest26\/methyl-cellulose\/","title":{"rendered":"Methyl Cellulose"},"content":{"rendered":"<p><strong>Method 1:<\/strong><br \/>\n3% aqueous Methyl cellulose:<br \/>\n1. Heat 100ml of DI water to 85\u00b0C<br \/>\n2. Shake 3g of Methyl Cellulose powder into the hot water and stir rapidly while cooling the solution to 5\u00b0C in an ice bath<br \/>\n3. Solution is stable at room temperature but store in tightly closed containers.<br \/>\n4. Use it to slow down protozoa for microscopy<\/p>\n<p><strong>Method 2:<\/strong><br \/>\nFrom : <a href=\"https:\/\/www.researchgate.net\/\" target=\"_blank\" rel=\"noopener\">https:\/\/www.researchgate.net\/<\/a><\/p>\n<p>I have made up to 4% methylcellulose perfectly clear in the following way.<\/p>\n<ul>\n<li>Take 1\/2 the volume of your liquid, heat to about 30 C in a vessel with a large stir bar. I use a 200 mL wide-mouth glass tissue culture bottle.<\/li>\n<li>Put the other 1\/2 volume of liquid in a container on ice to cool to 1-4 deg C..<\/li>\n<li>Take the WARM solution off the heat, add your MC with stirring. Manual stirring works best; I use a glass rod to go in and around the stir bar. The MC will not go into solution yet; it will be white and sticky with blobs.<\/li>\n<li>Now add your COLD solution, put on a stir plate and stir vigorously. You may have to stir a few times manually to loosen the stir bar. The stir plate must have a strong magnet to generate enough force. If it is too weak, stir by hand.<\/li>\n<li>Once most of the MC is in solution, refrigerate overnight. Bubbles will disappear and you will have a clear solution.<br \/>\nThe previous comment is correct that excess heat causes methylcellulose to gel, which may explain the cloudiness of the previous preparation.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Method 1: 3% aqueous Methyl cellulose: 1. Heat 100ml of DI water to 85\u00b0C 2. Shake 3g of Methyl Cellulose powder into the hot water and stir rapidly while cooling the solution to 5\u00b0C in an ice bath 3. Solution is stable at room temperature but store in tightly closed containers. 4. Use it to [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-2053","post","type-post","status-publish","format-standard","hentry","category-experiments"],"_links":{"self":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts\/2053","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/comments?post=2053"}],"version-history":[{"count":0,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts\/2053\/revisions"}],"wp:attachment":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/media?parent=2053"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/categories?post=2053"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/tags?post=2053"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}