For 18 to 20 plates:
\n5g commercial Nutrient Broth (a powder) from Southern Biological
\n(Or 1g Beef Stock Powder & 2g Peptone)
\n10g agar powder
\n500 ml distilled water.
\nWeigh agar. Add water, stir until dissolved.
\nAdd nutrient broth.
\nHeat carefully until boiling. Don\u2019t boil over.
\nPour into Schott bottles-with the blue cap. Put cap on loosely.
\n(Or pour into conical flasks and stopper loosely with cotton wool)
\nSterilise 20mins at 151 lb\/sq in. in an Autoclave or pressure cooker.
\nCool a little and pour into sterile petri dishes. using the \u2018Aseptic Technique\u2019.
\nSeal in clear plastic bags, in the fridge. Store upside down; this stops the media from getting wet.
\nOnce inoculated, the plates must be sealed around the edges with Parafilm or wide sticky tape or completely wrapped in cling wrap.
\nInoculated plates must not be reopened.
\nDo not dispose of contaminated plates straight into the bin.
\nThe Health Department insists that THE PLATES MUST BE RE-STRERILISED IN AN AUTOCLAVE OR PRESSURE COOKER BEFORE DISPOSAL.
\nIf this can\u2019t be done then the experiment has to be cancelled.
\nPlace into an oven bag, and re-sterilise as above. They are then safe to throw into the bin.<\/p>\n","protected":false},"excerpt":{"rendered":"
For 18 to 20 plates: 5g commercial Nutrient Broth (a powder) from Southern Biological (Or 1g Beef Stock Powder & 2g Peptone) 10g agar powder 500 ml distilled water. Weigh agar. Add water, stir until dissolved. Add nutrient broth. Heat carefully until boiling. Don\u2019t boil over. Pour into Schott bottles-with the blue cap. Put cap […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[31],"tags":[],"class_list":["post-385","post","type-post","status-publish","format-standard","hentry","category-technical-tips"],"_links":{"self":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts\/385","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/comments?post=385"}],"version-history":[{"count":0,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/posts\/385\/revisions"}],"wp:attachment":[{"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/media?parent=385"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/categories?post=385"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ciderhousetest.com\/labnetwest26\/wp-json\/wp\/v2\/tags?post=385"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}