Hydra culture:

Hydra is available from Southern Biological Services Pty Ltd, Victoria. Place Hydra in an 11cm petri dish or other glass or hard plastic container. Add about 2.5cm of Evian spring water. This seems to give the best results. It has been found that Hydra do not thrive in other types of spring water. Change the […]

Globes-memory aid:

Small globes come in 2V, 6V and 12V sizes. The filament colours of the ones we use are different and the 6V globes are bigger than the others, so I find it easy to tell the difference. I now have the following poem pinned up near the globes. GLOBES! Blue are 2 6 are fat […]

Glassware cleaning:

The list of cleaning solutions and methods for specific contaminants presented here should cover most of the glassware cleaning a technician may have to perform. If an automatic dishwasher is used a final rinse with distilled water may be required before drying. CLEANING SOLUTIONS: A. Basic permanganate solution. Dissolve 20g of potassium permanganate and 50g […]

Eyes-preservation

Question: Can you please tell me how to preserve eyes for dissection? Answer: Just keep them in isotonic saline (0.9% NaCl) in the fridge for up to one week. Change daily. If for longer, freeze them in the saline water.

Enzymes- recipes:

Diastase: Recommended working strength: 0.1 gram powder/100 ml 0.1% buffer Reaction buffer: 0.9% Sodium chloride, 0.01% Calcium chloride, pH7 Optimum temperature: 20 -25 degrees C Solution: always use fresh; will last for 12 hours at 4 degrees C Pepsin: Recommended strength: 0.1-0.5 gram/100 ml 0.1 -0.5% buffer Reaction buffer: 0.9% Sodium chloride at pH2; acidify […]

Electro-magnets:

Question: Has anyone a good design for electro-magnets that actually works? It seems the designs in the texts are rather weak and not very effective. Answer: In order to have sufficient current passing through the wire, the wire must be rated to carry at least 3 amps. Wire of this thickness can cause the electromagnet […]

Egg protein substitutes:

1.Try using gluten flour. It seems to react with the modified biuret reagent. Using 1M sodium carbonate with a couple of drops of 0.1M copper sulfate gives a violet precipitate for positive protein. 2.Try gelatine gels. They are about 90% protein (might be an idea to try some jelly cubes). 3.Try the egg replacement powder […]

Egg de-shelling:

Soak eggs in clear vinegar for around 1 to 3 days prior to use, making sure the eggs are completely covered. After soaking the first day, use gloves and remove any scum that has built up on the surface. Top up with vinegar or replace with fresh vinegar. Finally, gently buff the eggs with a […]